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Online Resumes with "cas9"
Summary * Designed and produced 32 knockout mutants using CRIPSR/Cas9 technology. * Specialist in cloning and molecular biology techniques. * Worked in multicultural teams in three different countries developing great flexibility to adapt to different environments to fluent oral and writing in skills in three different languages. * Excellent problem solving and independent thinking, which led to acquirement of grants from b...
* Successful research professional with 6+ years' postdoctoral experience * Exceptional knowledge of molecular cancer biology * Expertise in multidisciplinary cancer drug discovery and development * Hands on experience with in vivo tumor xenograft, orthotopic and metastatic models, molecular techniques and cell based assays * Versed in organizing, writing and editing detailed reports and research manuscripts * Excellent int...
SUMMARY >12 years hands-on experience in nucleic acids and molecular genetics (nucleic acids extraction/purification/amplification/hybridization, molecular cloning & mutagenesis, in vitro transcription, DNA sequencing, genomics, transcriptomes) >10 years hands-on experience in cell biology (cell culture, stable cell line generation through transfection/transduction/CRISPR-CAS9 editing) >3 years hands-on experience with Next...
and professional experiences
Tags for this Online Resume: VASCULAR, Cancer, Critical Care - Vascular, Microbiology, Pulmonary, Research, ELISA, Genotyping, Molecular Biology, CRISPR/Cas9, Cloning, Bioinformatics, Protein synthesis, Fluoresence confocal microscopy, FRET Microscopy, Aisotropy, CRISPR imaging
Experienced with CRISPR CAS9 and knockout mice. Purified recombinant proteins including v-src, MAP kinase, MEK and full length nuclear receptors. Experienced with protein expression and purification using Pichia pastoris, E. coli and baculovirus expression systems. Established and maintained primary cell cultures from human, avian, and murine tissues. Experienced in culturing mammalian cell lines. Experienced in molecular b...
Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors, DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT-qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his-tag, flag-tag, flash-tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR-technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells
Summary 1. Two-years of experience in cancer research 2. Deep expertise in molecular and cellular biology: after pre-PhD training in Japan and having been working for fifteen years in the United States in the molecular and cellular laboratory setting, highly experienced in molecular cloning, quantitative real-time PCR, western blotting, immunohistochemisty, co-immunoprecipitation (co-IP) for protein-protein interactions, ch...
Highly motivated and innovative research biologist with extensive experience and expertise in molecular and cell biology biochemistry proteomics and gene editing technology Experienced in managing and conducting multiple projects simultaneously Expertise in development and execution of cell and genetics based research methods Enthusiastic team player adept at providing leadership while also learning from fellow team members...
Tags for this Online Resume: Cellular, Research, Western blot, High Performance Liquid Chromatography (HPLC), Data Analysis, Engineering, Microsoft Excel, Microsoft PowerPoint, Adobe, Adobe Photoshop, research, Gene editing technology, CRISPR/Cas9 and RNAi technology, Molecular cloning, DNA, RNA isolation and purification, PCR, qRT-PCR, DNA sequencing, Mammalian cell culture and transfection, Cell line engineering and development, Cell-based phenotype screening, Protein expression and purification in bacterial and mammalian system
AREAS OF EXPERTISE * Cancer Biology * In vivo disease models * Research & Analysis * Molecular Biology * Genome Editing * Scientific Reporting * Cell Biology * Cell-Based Assays * Scientific Communications * Stem Cell Biology * Histology & Microscopy * Team Leadership Technical & Laboratory Skills: Mammalian Cell Culture * Retroviral & Lentiviral Transduction * Biochemical Assays * RNAi CRISPR / Cas9 * Proliferation/Apoptos...
PROFILE Virologist/molecular biologist with over 15 years post-doctoral/research scientist experience in multiple research projects using a wide variety of molecular biological and cell culture techniques. * Cell culture and growth of pathogenic organisms in culture. * Experience with nucleic acid isolation/amplification of virus isolates from tissues and infected cell culture. Experienced with procedures and personal prote...
Tags for this Online Resume: Cellular, Bonds, Collection, Control Data, ELISA, Microsoft Excel, Microsoft PowerPoint, Microsoft Word, Realtime, Research, virology, molecular biology, cell culture, BSL-3, cell biology-cancer, biosafety
Qualifications Overview * Well qualified Research Scientist with over 10 years of laboratory experience and outstanding academic qualifications * Extensive experience in state of the art research techniques including mammalian cell culture, mouse xenograft model, CRISPR/Cas9, real-time RT-PCR, RNA/DNA isolation, protein isolation, immunoprecipitation, Western blot, immunofluorescence, immunohistochemistry, microscope (light...